| Arukas Kumagaya |
| Blue Angels |
| Fukuoka Ladies RFC |
| Hanazono Hollyhocks |
| Hokkaido Barbarians Diana |
| Ishikawa Nadeshiko RFC |
| Katagami Iris |
| Kyoto Joinus |
| Mie Womens Rugby Club Pearls |
| Nagasaki Ladies RFC |
| Nagoya Ladies RFC |
| Naniwa Rugirls |
| Neyagawa Womens |
| Nippon Sports Science University Women's Rugby Club |
| Oita Women's RFC |
| Okayama Rugby Girls |
| ORS Ladies |
| Pegasus Rugby Club |
| Red Coral |
| RKU Rugby Ryugasaki GRACE |
| Rugirl-7 WEST |
| Ryukyu Island Girls RFC |
| Shiga Girls Rugby Club Breeze |
| Shonan Bellmare Bell 7 Ladies |
| Sweetie Lady Bears |
| Tokushima Seven Series |
| Tokyo Phoenix RC |
| Yokogawa Musashino Artemi Stars |
| Yokohama TKM |
